ISOLATION AND IDENTIFICATION OF FUNGI ASSOCIATED WITH SLICED WATERMELON AND PINEAPPLE SOLD IN USMANU DANFODIYO UNIVERSITY, SOKOTO

Abstract

ABSTRACT This study investigated the fungi associated with sliced watermelon (Citrullus lanatus) and pineapple (Ananas comosus) sold within Usmanu Danfodiyo University, Sokoto, with the aim of assessing their potential public health implications. Six samples of sliced fruits were collected from vendors within the campus and analyzed using standard microbiological techniques on Potato Dextrose Agar (PDA). Fungal isolates were identified based on their macroscopic and microscopic characteristics. The results revealed six fungal species: Rhizopus oryzae, Saccharomyces cerevisiae, Scopulariopsis brevicaulis, Aspergillus niger, Aspergillus flavus, and Aspergillus oryzae. Saccharomyces cerevisiae had the highest frequency of occurrence (40%), while Aspergillus flavus and A. oryzae showed the least (10% each). Rhizopus oryzae was isolated only from pineapple, whereas the Aspergillus species were found mainly in watermelon. The presence of Aspergillus flavus, a known aflatoxin producer, highlights a potential risk of mycotoxin exposure among consumers of sliced fruits sold under unhygienic conditions. The study concludes that inadequate handling practices, exposure to air, and lack of proper storage significantly contribute to fungal contamination. It recommends improved hygiene among fruit vendors, enforcement of food safety regulations, and increased public awareness to minimize health risks associated with the consumption of contaminated sliced fruits.